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Joshi, Chinmayi
- Influence of a Mono-Frequency Sound on Bacteria can be a Function of the Sound-Level
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Authors
Vijay Kothari
1,
Chinmayi Joshi
1,
Pooja Patel
1,
Milan Mehta
1,
Sashikant Dubey
1,
Brijesh Mishra
1,
Niral Sarvaiya
1
Affiliations
1 Institute of Science, Nirma University, Ahmedabad – 382481, Gujarat, IN
1 Institute of Science, Nirma University, Ahmedabad – 382481, Gujarat, IN
Source
Indian Journal of Science and Technology, Vol 11, No 4 (2018), Pagination:Abstract
Objectives: To investigate the effect of a mono-frequency (300 Hz) sound on the bacterium Chromobacterium violaceum at different sound levels. Methods: Chromobacterium violaceum was subjected to sonic stimulation with 300 Hz sound, at five different levels of loudness in the range of 70–89.5 dB. Effect of sound treatment was studied on cell yield and pigment production of this bacterium. Findings: Sonic stimulation was found to affect bacterial growth and quorum sensing regulated pigment (violacein) production significantly. Magnitude of this effect was found to be dependent on sound-level. The minimum critical difference required to cause any statistically significant change in bacterial response with respect to sound-level was found to be 13 dB. Growth of C. violaceum was affected more at lower sound intensity, whereas pigment production was affected more at higher sound intensity. Additional experiments with C. violaceum and Serratia marcescens indicated that even a silent speaker emitting no sound can alter bacterial growth and/or pigment production up to a minor extent. Size of the test tube in which bacteria are exposed to sonic stimulation was not found to affect the results much. Application: This study has shown that C. violaceum does respond to sonic stimulation and that the intensity of sound is an important determinant affecting magnitude of this response.Keywords
sonic stimulation, violacein, prodigiosin, quorum sensing, sound level, magnetic field effectFull Text
- Frequency-Dependent Response of Chromobacterium violaceum to Sonic Stimulation and Altered Gene Expression Associated with Enhanced Violacein Production at 300 Hz
Abstract Views :273 |
PDF Views:74
Authors
Affiliations
1 Institute of Science, Nirma University, Ahmedabad 382 481, IN
1 Institute of Science, Nirma University, Ahmedabad 382 481, IN
Source
Current Science, Vol 115, No 1 (2018), Pagination: 83-90Abstract
In this study, Chromobacterium violaceum was subjected to sonic (100–2000 Hz) stimulation. Sound waves of 300 Hz frequency promoted bulk production of the quorum-regulated pigment, violacein. Whole transcriptome analysis indicated that a total of 342 genes (i.e. 4.63% of the whole genome) were significantly upregulated in the sonic stimulated culture. Enhanced violacein production in the sound-stimulated culture seems to have stemmed from enhanced expression of the genes involved in pentose phosphate pathway, resulting in an increased availability of erythrose-4-phosphate to be used in the synthesis of tryptophan – the precursor of violacein synthesis. This study is a good demonstration of the ability of sound waves to alter bacterial metabolism.Keywords
Altered Gene Expression, Chromobacterium violaceum, Sonic Stimulation, Violacein.Full Text
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